This paper reports on research to develop local indicators of ‘habitation comfort’ that are relevant for population health in the Vladimir region, located in Central Russia. We present a conceptual model based on the literature which led us to include three groups of factors for assessing population habitation comfort: natural-anthropogenic, social and medical-ecological. To assess and display a ‘comfort map’ of socio-economic conditions, a single database has been developed Higher comfort level is associated with better social-economic conditions, and also with proximity to major economically developed regions like Moscow and Nizhniy Novgorod. The lowest comfort level index was detected in the regions with the worst natural-anthropogenic and social-economic conditions. In further analysis, demographic conditions in the Vladimir region were evaluated by administrative region, and medical-ecological zoning was carried out based on principal causes of diseases affecting the population.